TriniCHROM Antithrombin IIa and TriniCHROM Antithrombin Xa

TriniCHROM Antithrombin IIa and TriniCHROM Antithrombin Xa are intended for the quantitative determination of AT activity in human plasma by chromogenic assay.

AT is the major inhibitor of plasma thrombin and Factor Xa. It is also an important inhibitor of activated Factors IXa, XIa, and XIIa. The inhibitory activity of AT towards thrombin is greatly increased (2 3 orders of magnitude) in the presence of heparin. TriniCHROM Antithrombin IIa and TriniCHROM Antithrombin Xa utilize a thrombin based reagent which is added to a plasma dilution containing AT in the presence of heparin. After incubation, residual thrombin is determined with a thrombin-specific chromogenic substrate. The residual thrombin activity is inversely proportional to the antithrombin concentration.

Adapted to all clinical contexts:

Allows for the detection of both types of AT deficiencies (type I, II) Wide working ranges & the choice of using the IIa or Xa method: ✓ ✔ The use of bovine thrombin and low heparin levels reportedly reduce

the interference of HCII in amidolytic assays. This AT assay has been developed to decrease the interference from HCII to a level where discrimination between normal and abnormal levels is similar to that achieved by Factor Xa based AT assays.

✓ ✔ A method based on the ability of plasma to inhibit Factor Xa in the presence of heparin may be more accurate since it eliminates interference due to heparin cofactor II which does not inhibit Factor Xa.

TriniCHROM Speciality Reagents

PART NUMBER PRODUCT NAME PACKAGING STABILITY

T2602 TriniCHROM Antithrombin IIa

AT Heparin/Thrombin Reagent: 4 x 12 mL AT Thrombin Substrate: 4 x 2 mL AT IIa Dilution Buffer (10X): 2 x 5 mL

AT Heparin/Thrombin Reagent: 2 weeks at 2-8°C AT Thrombin Substrate: 2 weeks at 2-8°C AT IIa Dilution Buffer: 2 weeks at 2-8°C

T2603 TriniCHROM Antithrombin Xa

AT Factor Xa Reagent: 4 x 3 mL AT Factor Xa Substrate: 4 x 3 mL AT Xa Dilution Buffer (10X): 4 x 5 mL

All reagents are stable for 1 month at 2-8°C

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