TRAUMEEL® PRODUCT MONOGRAPH22

Pre-clinical studies

Actions of Traumeel® Broad range of anti-inflammatory and immunomodulatory effects in vitro

and in vivo.[St Laurent 2017, St Laurent 2021, Jordan 2021]

Wound healing and antioxidative effects were also demonstrated in animal models.[St Laurent 2017]

Significant edema volume reduction of up to 15% seen with Traumeel® in carrageenan-induced edema test.[Conforti 1997]

A significant reduction in acute local inflammation (first phase of adjuvant arthritis) in adjuvant arthritis model in rats.[Conforti 1997]

At 3 and 5 hours post intra-paw injection of a small amount of homologous blood, local inflammation was significantly lower in rats treated with Traumeel® compared with controls.[Lussignoli 1999]

Levels of IL-6, a pro-inflammatory cytokine, were significantly reduced by 45% in Traumeel®-treated rats compared with controls at 5 hours post blood injection.[Lussignoli 1999]

Traumeel® may reduce muscle damage and inflammatory response following an acute bout of eccentric exercise. [Muders 2016]

Traumeel induces differentiation of primary human skeletal muscle cells. [Langendorf 2019]

Traumeel® accelerates inflammation resolution demonstrated by faster reduction of inflammatory cells, polymorphonuclear leukocytes (PMNs) in an animal model.[Jordan 2021]

In silico research/bioinformatics New technologies allow us to study networks of inflammation resolution. In silico is the term scientists use to describe the modeling, simulation and visualization of biological and medical processes in computers. In silico models can integrate various types of data, including DNA, RNA, protein, metabolic products and more. In silico models link processes to clinical outcomes or other endpoints, such as biomarkers and predictive markers. They have an important role in toxicology studies, as well as other areas.

Wound-healing model: transcriptomics While DNA remains static, RNA is constantly changing. The transcriptome is the set of all RNA molecules, including mRNA, rRNA, tRNA, and other noncoding RNAs produced in the cell. Unlike the genome, RNA is able to vary under the influence of external environmental conditions.[Kahl 2015, Manzoni 2018, Berg 2020] Studying transcriptome changes is an ideal tool to analyze drug effects.[Manzoni 2018, Debnath 2010]

In the St Laurent et al. study Traumeel® produced biologically significant and consistent changes in hundreds of gene ontologies involved in wound healing.[St Laurent 2017]