TRAUMEEL® PRODUCT MONOGRAPH24

Traumeel® ointment and injection have common and specific effects on gene expression, offering additional rationale for the different administration routes.[St Laurent 2017]

Traumeel s effects on gene expression are consistent with its established effects on inflammation and pain.[St Laurent 2017]

Zymosan-induced peritonitis model The zymosan-induced peritonitis model is a self-limited resolving model of acute inflammation with a defined time frame. It enables the quantification of the recruitment and reduction of monocytes and neutrophils into the peritoneal cavity. The time taken to achieve a reduction of 50% in neutrophils is indicative of the speed at which resolution occurs. The model mimics events happening in vivo, however the duration of individual steps needs to be considered with care when knowledge is transferred to humans and outside of a self-limited model.[Cash 2009, Serhan 2018]

Findings In the recently published in vivo study by Jordan et al. the zymosan-induced murine peritonitis model was used to assess the capacity of Traumeel® to promote inflammation resolution. The results show that Traumeel® supported the recruitment of innate leukocytes and the efferocytotic capacity of macrophages, and positively influenced the inflammation resolution index. The authors suggest that based on these properties, Traumeel® may possess therapeutic potential as an enhancer for the resolution of inflammatory processes.[Jordan 2021]

Traumeel® shortened the the resolution interval (Ri interval between Tmax and T50) with the high dose by 1.3 hours and with the low dose by 5.9 hours. The clearance of neutrophils out of the inflamed area is a major milestone in

the resolution of inflammation. The resolution interval is thus indicative of the rate of neutrophil clearance

which has drastically been improved by Traumeel®.

Po ly

m or

ph on

uc le

ar le

uk oc

yt es

(P

M N

) ( ×

10 6 )

10

8

6

4

2

0 0 2016128

Time (hours)

Ri = - 5.9 h

∆Ri

*

1.5 mL/kg Traumeel®

Vehicle

* p < 0.01Ψ max

T max T 50 T 50

Ψ 50

Ri ~ 5.6 Ri ~ 11.5

244

Figure 6 Effects of Traumeel® pre-administered i.p. over 6 days prior murine peritonitis induction, on resolution of inflammation[Jordan 2021]